G-418, neomycin, kanamycin and gentamicin

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Wed Jul 15 11:31:57 EST 1998


Eugene <ronlab at uic.edu> wrote:
> Note that G418 is also called neomycin (!) sulfate and in a number of vector
> systems the same marker is used to select bacteria on kanamycin and
> eukaryotes on G418. The resistance marker is called neomycin (!)
> phosphotransferase or simply "neo".
> Gentamicin is often used as a supplement in mammalian cell culture to
> suppress bacterial growth. Thus, I doubt that it is efficient in selecting
> mammalian cells for neo - resistance. I have seen people mistaking it for
> "Geneticin" (another name for G418)

Duh. Seems I messed up in two ways: first, confusing "Geneticin" with
"Gentamicin", and second forgetting about that "neo" resistence (although
I am sometimes working with pSV2Neo, which should have taught me).

Sorry,
--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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