Best way to store DNA?

Jakob Begun jb261 at cryst.bioc.cam.ac.uk
Wed Jul 15 09:25:41 EST 1998


John Ladasky wrote:
> 
> In article <6oh6tc$ocj$1 at mark.ucdavis.edu>,
> Anne Gillen <ez049617 at dilbert.ucdavis.edu> wrote:
> >I would store it as a dry pellet for long term storage.
> 
>         For long genomic DNA, this could be a very bad idea.  Have you
> ever tried to resuspend genomic DNA that you left on the Speed-Vac for
> too long?  If it goes into solution at all, it takes days.
> 
> >I do not know if
> >it matters what temp a dry pellet is stored at.  Synthesized oligos are
> >sent at RT using FedEx.  However, the manufacturer recommeds long term
> >storage of dried  oligos at -20C.  DNA in water is subject to acid hydrolysis,
> > as well as nuclease activity.
> >
> >I always hydrate with TE pH 8. Degradation is TE is slower than water.
> >Does anyone know why?  My genomic DNA is just fine at 4C, but I have only
> >a little experience with plasmids.
> 
>         You started to explain it above.  DNA in water is subject to acid
> hydrolysis.  DNA is acidic, and can catalyze the hydrolysis of its own
> purine moieties, albeit rather slowly.  The TE, being buffered to pH 8,
> prevents this acid-mediated hydrolysis.
> 

The EDTA in TE buffer also inhibits nuclease activity...



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