CTAB-agarose gel electrophoresis for cationic proteins

tonyho s970856 at CUHK.EDU.HK
Thu Jul 16 22:50:29 EST 1998


Hi everyone,

     Does anyone knows about and have experience in doing agarose (with
0.02% CTAB, a cationic detergent called cetyltrimethylammonium bromide) gel
electrophoresis for cationic proteins, like those of granular proteins from
polymorphonuclear granulocytes? A paper suggested using Pharmacia
IEF-Agarose gel to perform a 2-D Zone electrophoresis. Has anybody done that
before?

Thanks for giving me some advice !!! :)

Tony Ho (Ph.D student)





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