Best way to store DNA?

Hiranya Roychowdhury hroychow at NMSU.EDU
Fri Jul 17 10:59:09 EST 1998


At 06:36 PM 7/16/98 GMT, Conrad R Fjetland wrote:
>
>: DNase, if present in the sample, will degrade DNA, albeit slowly, even at 4
>: C. In fact, I had done a test, following a similar comment by one of our
>: grad students. I added some DNaseI to three equal aliquotes of a plasmid
>: prep that I knew was quite free of any. One of the aliquotes I incubated on
>: the bench, the second was incubated at 4 C (cold rm) and the third was
>: stored at -20. The plasmid in the tube at RT was all but gone after an hour.
>: The tube stored at 4 C had all its DNA degraded after 5h and the tube at -20
>: showed the presence of plasmid for about a week (with smearing below the
>: band), the final gel showing no trace of any plasmid. In the case of the -20
>: tube, it is hard to infer if the DNaseI was active at that temp. The
>: degradation could have been during the daily thawing of the sample for the
>: monitoring.
>:         No, I did not do this in triplicates.   
>
>
>: Dr. Hiranya Sankar Roychowdhury
>: Plant Genetic Engineering Lab.
>: New Mexico State University
>: Las Cruces, NM 88003
>: Ph. (505) 646-5785
>: hroychow at nmsu.edu
>
>Hello PGEL,
>
>Say hello to Suman and Dr. Gopalan for me.  I just got my Ph.D. from NMSU.
>I worked with Dr. Lammers.  Nice to know there are more Aggies around.
>
>Conrad
>
>

Hello Conrad,
        Thanks for the note. Suman says hello too. Champa is on vacation
(somewhere in BC, I believe). How and what are you doing presently?

Hiranya.


Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu




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