Optimal Electroporation settings for JM109

Tam Anh Le leta at svhm.org.au
Sat Jul 18 00:30:14 EST 1998


Hello all
    I have always used heat shock as a methods of transforming bacterial
cell with my desired plasmid, however my supervisor has told me that
electroporation is a more effective than heat shock. To that extend I want
to try this method of transformation, however I do not have the information
regarding the optimal setting host strain JM109, I am hoping someone out has
this information and could share it with me.

-Many thanks

--
Tam Anh Le
Clinical Neuroscience
St Vincent Hospital Melbourne.





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