microarrays and total RNA

ELADER at AMBION.COM ELADER at AMBION.COM
Fri Jul 17 17:16:38 EST 1998


In article <35AB67E9.1951 at ucla.edu>,
  dtyyu at ucla.edu wrote:
> The Clontech and the GenomeSystems microarray of cDNA/EST recommend
> using mRNA for generating probes.  Has anyone tried using total RNA
> instead in case samples cannot provide enough mRNA?
>
> Best wishes,
> David Yu
> --

if ya gotta, ya gotta. Use anchored oligo dt primer (dt-a/g/c) this should
help avoid both long stretches of polyA and priming off of ribosomal.

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