Help asked

[wuzi]

Hi: Net-friends:

I am trying to Screen tobacco cDNA library using PCR directly by
designing a pair of specific internal primers (of my interest) and 5' or
3' insert sreening primers.  I did get a expected size of PCR product
with the pair of internal primers, however, when I used the specific
primer in combination with the vector primer, the PCR band is vary
faint and repeatibility of the PCR reaction is very low.  Does anybody
has any tip or comments on direct PCR screening of Lambd gt11 library?
and your input will be greatly appreciated.

Hsieh

wuzi at onramp.net


Wuzi Xie, Ph.D.
BioTex
500 E. 50th Street
Lubbock, TX 79404
Phone: 806-747-6952
Fax:   806-747-3044