PCR on repeated sequences

mbarnhart at my-dejanews.com mbarnhart at my-dejanews.com
Tue Jul 21 11:56:19 EST 1998


Hi,

We want to do quantitative PCR on polyubiquitin.  This gene contains ten
repeats coding for 76 amino acids.  The repeats are identical or have 1 base
change. Does anyone have any experience doing PCR under such circumstances
and can we do accurate quantitation using PCR on this gene?

We have about 160 nt from the poly A signal to the end of the repeated
sequences, but I'm not sure this is a large enough segment.

Any ideas will be greatly appreciated.

Thanks,
Michael

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