RbCl Procedure for Competent E. coli

Sebastian Bunka bunka at i112pc09.vu-wien.ac.at
Tue Jul 21 06:58:38 EST 1998


John G. Wise <wise at chemie.uni-kl.de> wrote:
> Does anyone have a reference for a RbCl Procedure for making competent
> E. coli?  Our lab has been using a photocopied page out of a catalog -
> the procedure works extremely well, but there is no citation and I don't
> know who to cite!! At the bottom of the page it mentions Joseph
> Utermohlen of the University of Arizona, but this has not brought me any
> closer to finding a reference.

> If anyone has any suggestions as to where to start looking, please
> forward them to,

Hanahan, D. 1983, J. Mol. Biol. 166:557

Check also Sambrook, Fritsch, Maniatis 2nd edition pp. 1.76-1.81
Sambrook suggests KCl instead of rubidium chloride.

SWB
-- 
Sebastian Bunka, Dr. med. vet
Inst. Med. Chemistry, Vet. University Vienna
Ph. +43-1-250 77 ext. 4208, Fax: ext. 4290
e-mail: Sebastian.Bunka at vu-wien.ac.at



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