Q:Extracting TCA Ppted Proteins

Mr. Chirag Joshi chirag at cge.iisc.ernet.in
Wed Jul 22 05:38:09 EST 1998


Hello!
	Would anyone have an idea as to how could the proteins
precipitated on glass fibre membrane, with TCA, could be extracted (say
for SDS PAGE)? I have tried solubilizing in 6M urea/ SDS-PAGE loading dye but
that didn't help. 
Thanks for any inputs.

CJ
______________________________________________________________________________
Chirag V. Joshi
Department of Microbiology and Cell Biology
Indian Institute of Science
Bangalore 560 012, India

Phone : ++91-080-309 2344, -334 4668
Fax   : ++91-080-344 4697, -334 1683

Email : chirag at cge.iisc.ernet.in
Internet: http://144.16.78.192/~chirag/
______________________________________________________________________________




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