Success with Anti-His antibody from CLONTECH ?

Yves Durocher yves.durocher at nrc.ca
Wed Jul 22 09:15:40 EST 1998


Does anyone succeeded in detecting C-terminal 6xHis-tagged proteins by
Western blot using Clontech's anti-histidine antibody (cat#8904-1) ?

I have transiently expressed a myc/his-tagged protein but I don't see a
damned signal using an ECL detection system (30 minutes exposure) while
using an anti-myc antibody (Invitrogen), strong signal is seen within 20
seconds of exposure.

Thanks for your advises,

Yves



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