How to PAGE purify oligos?
gruidlme at moffitt.usf.edu
Wed Jul 22 07:10:25 EST 1998
Another way is to do UV shadowing. Basically use denaturing PAGE to
size fractionate the entire oligo sample, use a minigel or protein gel
for this. Transfer gel to plastic wrap. Place wrapped gel on a
fluorescent TLC plate and use a hand held UV light to visualize the dark
non-fluorescent area where your oligo is on the gel. Mark with a pen
and cut out the gel piece. Elute the oligo in buffer overnight at 37C.
Precipitate and suspend in the buffer you want. Usually you get about a
50% yeild. Usually someone in a department will have a few plates
around. One plate will last you a long time. This works for RNA as
well when making riboprobes.
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