RbCl Procedure for Competent E. coli

Rafael Maldonado rmaldonado at jamonua.es
Wed Jul 22 07:22:51 EST 1998


John G. Wise <wise at chemie.uni-kl.de> wrote:
> Does anyone have a reference for a RbCl Procedure for making competent
> E. coli?  

RbCl inhibits transformation!! It is totally unuseful. Check for the
best transformation protocol:

Gene 1990 Nov 30;96(1):23-28 
High efficiency transformation of Escherichia coli with plasmids.
Inoue H, Nojima H, Okayama H


-- 
Rafael Maldonado
Division of Genetics
University of Alicante
rmaldonado at jamonua.es 
(don´t use "jamon" to contact me)



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