Q:Extracting TCA Ppted Proteins

Ian McFarlane i.mcfarlane at icrf.icnet.uk
Wed Jul 22 06:15:44 EST 1998


CJ

TCA precipitated proteins will redissolve in 1M Tris pH10.4. I would leave
your filters in a small amount of this solution for a couple of hours and
see what you get back.

Ian Mc






In article <6p4feh$77u at mserv1.dl.ac.uk>, "Mr. Chirag Joshi"
<chirag at cge.iisc.ernet.in> wrote:

> Hello!
>         Would anyone have an idea as to how could the proteins
> precipitated on glass fibre membrane, with TCA, could be extracted (say
> for SDS PAGE)? I have tried solubilizing in 6M urea/ SDS-PAGE loading dye but
> that didn't help. 
> Thanks for any inputs.
> 
> CJ
> ______________________________________________________________________________
> Chirag V. Joshi
> Department of Microbiology and Cell Biology
> Indian Institute of Science
> Bangalore 560 012, India
> 
> Phone : ++91-080-309 2344, -334 4668
> Fax   : ++91-080-344 4697, -334 1683
> 
> Email : chirag at cge.iisc.ernet.in
> Internet: http://144.16.78.192/~chirag/
> ______________________________________________________________________________



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