ethanol precipitation of RNA-Help!

Peter pxpst2 at SPAM.SUXS.unixs.cis.pitt.edu
Wed Jul 22 14:51:28 EST 1998


In article <35B623BA.7E67 at olive.ls.biu.ac.il>, ilya at OLIVE.LS.BIU.AC.IL wrote:

> Hi,
> 
> I did precipitate RNA with 0.1V 3M NaAc(5.2) and 2V (!) ethanol (100%).
> Is it posible to precipipitate whole RNA with only 2V (not 2.5V) of
> ethanole? It is critical experiment.

as little as 1.5 volumes of ETOH is needed.  2.5 volumes will not change
RNA precipitation significantly but you may get more salt contaiminant.
Also, one can use 1 vol of isopropanol(2-propanol)

Peter

-- 
"Don't you eat that yellow snow
            watch out where the Huskies go"    FZ

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