ethanol precipitation of RNA-Help!

Patrick F.H. Lai pfhlai at LOOKSMART.COM
Thu Jul 23 00:28:40 EST 1998


---- Begin Original Message ----
Hi,
I did precipitate RNA with 0.1V 3M NaAc(5.2) and 2V (!) ethanol 
(100%).
Is it posible to precipipitate whole RNA with only 2V (not 2.5V) of
ethanole? It is critical experiment.

Thanks in advance.
Ilya
---- End Original Message ----

Dear Ilya,

I think precipitating for a longer time would help.
Is this after phenol/chloroform extraction ?
How about using a smaller volume of choroform, so that the total 
volume with the NaAc & EtOH can be smaller, too ?






Hope this is useful info.   :-)


Patrick F.H. Lai  
Graduate Student, University of Toronto 
< PFHLai at looksmart.com >




      
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