ethanol precipitation of RNA-Help!

Patrick F.H. Lai pfhlai at LOOKSMART.COM
Thu Jul 23 00:28:40 EST 1998

---- Begin Original Message ----
I did precipitate RNA with 0.1V 3M NaAc(5.2) and 2V (!) ethanol 
Is it posible to precipipitate whole RNA with only 2V (not 2.5V) of
ethanole? It is critical experiment.

Thanks in advance.
---- End Original Message ----

Dear Ilya,

I think precipitating for a longer time would help.
Is this after phenol/chloroform extraction ?
How about using a smaller volume of choroform, so that the total 
volume with the NaAc & EtOH can be smaller, too ?

Hope this is useful info.   :-)

Patrick F.H. Lai  
Graduate Student, University of Toronto 
< PFHLai at >

LookSmart =85 or keep looking.

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