carrier DNA

Frederik Boernke boernke at
Fri Jul 24 11:28:34 EST 1998

Q. Guo wrote:
> Hi, you all.
> Can I use the S. Sperm DNA which is sheared for Southern blot in two
> hybrid's cotransformation?  It cost me $165 to just buy the
> sonicated carrier DNA from Clontech.
> Thanks.


I would say it depends on the average fragment size of your DNA which is
critical for high efficiency. It should be between 2 and 15 kb
(see Schiestl and Gietz, Curr Genet (1989) 16: 339 - 346). If it is not
just prepare your own carrier DNA it's pretty easy in my hands works 
even better than the commercial ones.

Good Luck


Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515

More information about the Methods mailing list