low LPS E.coli

Frank O. Fackelmayer fof1 at chclu.chemie.uni-konstanz.de
Fri Jul 24 10:36:52 EST 1998


I know it´s not exactly what you asked for, but what about passing your
supernatant through a column that efficiently removes LPS (e.g. Detoxi-Gel
from Pierce (no affiliation)). 

Frank



Lynn Hughes wrote:
> 
> Hi all,
> 
> I am trying to find some information about a low LPS content E.coli that
> could be used for expression.  I'm wanting to express my protein and use
> a crude supernatant as a vaccine, I have tried this with some success,
> but had some problems with LPS.  I am told there are low LPS strains
> around but cannot find any information about them.  Any help with this
> would be appreciated.
> 
> Lynn
> 
> Lynn Hughes
> Dept Biochemistry & Molecular Biology
> James Cook University
> Townsville, Queensland
> Austalia
> 
> email: lynn.hughes at jcu.edu.au



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