genomic DNA in plasmid prep

fwhyde at ibm.net fwhyde at ibm.net
Fri Jul 24 10:29:11 EST 1998


John Dixon wrote:

> > This may not work on Sundays or Tuesdays or whenever your time is
> most
> > pressed. :) I've yet to find any way of ridding samples of
> chromosomal
> > once its in the prep. (Tried some acid phenol, which can work if the
>
> > plasmid is not also removed.)
> >
> > If you can solve this, you will be a hero. (Plese E-mail me if you
> have
> > a clue).
>
> Has anyone tried the PLasmid-safe enzyme from Epicentre I think? I got
> a
> flyer about it once. They have some sort of exonuclease that digests
> linear dsDNA and closed circular ssDNA and linear ssDNA, but can't
> touch
> nicked/closed-circular/supercoiled DNA.

Epicentre's Plasmid-Safe product number E3101K (1000 U), E3105K (5000
U), E3110K (!0,000 U).




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