refolding on IMAC-column

Christian Ebeling cebelin at
Fri Jul 24 04:09:47 EST 1998

Hi netters,
i want to refold a recombinant produced protein on an IMAC column by
decreasing step-by-step the urea concentration. In some papers I have
read that it´s important to avoid high protein concentartions. But this
is the case when I bind my protein to the gel matrix. Have anyone of you
experience with refolding on a IMAC column?

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