Thermocyclers - Your opinion

Colin Rasmussen colin at nospam.fungus.usask.ca
Tue Jul 28 14:57:59 EST 1998


In article <6pkaie$qnl at cantine.wu-wien.ac.at>, Sebastian Bunka
<Sebastian.Bunka at vu-wien.ac.at> wrote:

> John Ladasky <jladasky at pmgm.Stanford.EDU> wrote:
> > >> 
> > >> I have heard that even if it does cool to 4 degrees, that it is not a
> > >> good idea to have the machine try to sustain that temperature for long
> > >> periods of time (eg., overnight).  It is supposed to cause more wear
> > >> and tear on the machine because it is actually designed to sustain
> > >> temperatures higher than room temp most of the time and uses the
> > >> cooling for achieving rapid changes in temperature, not to keep things
> > >> below room temp.  Is this true??  

We've got an MJ Research cycler that's about 5 years old now and we
routinely setup reactions int he afternoon and they wind up holding at 4°C
for 10 hrs or so.  No problems yet.

Colin Rasmussen



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