sheared libraries

Aippersbach Nicole C 3nca1 at qlink.queensu.ca
Wed Jul 29 10:36:16 EST 1998


We want to make a sheared genomic library and were wondering if it is
better to use linkers or just blunt end the gDNA and ligate to a blunt
site
in the vector.  Any input on sucesses with library construction
particularly in Drosophila is very welcome.

Thanks

Nicole



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