Ligation problems

[Patrick F.H. Lai]

Dear Ian,

One more thought:
Is the gene lethal to the bacteria ?

Pat.Lai
<PFHLai at looksmart.com>
Grad. Student, U of Toronto


---- Begin Original Message ----
We're having a terrible time getting what should be a straightforward
ligation to work.  I have a feeling we neglecting something obvious, so=
 I
won't be offended at simple suggestions ...

We have purified some genomic DNA and we want to put it into an express=
ion.....


Hope this is useful info.   :-)


Patrick F.H. Lai  < PFHLai at looksmart.com >
Graduate Student
University of Toronto
Toronto, Ontario, Canada





       
LookSmart =85 or keep looking.
http://www.looksmart.com
---- End Included Message ----




Hope this is useful info.   :-)


Patrick F.H. Lai  < PFHLai at looksmart.com >
Graduate Student
University of Toronto
Toronto, Ontario, Canada





       
LookSmart =85 or keep looking.
http://www.looksmart.com