SDS and Ni2+ resin

Vladimir Rotrekl rotrekl at
Thu Jul 30 05:47:27 EST 1998

Ian McFarlane wrote:

> What effect does SDS have on Ni2+ metal affinity columns. Does it
> merely
> interfere with the binding of the protein to the column or does it
> have
> some irreversible effect which would lead to the column being useless?
> Ian Mc

   It depends on resign, but generally no damage should be detectable in
low SDS concentration.
For example Ni-NTA agarose and superose (from Qiagen) require for
regeneration 2% SDS (recommended by manufacturer).

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