DNA extraction.

Deepali Mitra dmitra at HORT.CRI.NZ
Thu Jul 30 16:58:02 EST 1998


Hello
I have tried to extract DNA from tissue cultured tobacco leaves that
were soaking in 70-95 % ethanol in order to get rid of all the
chlorophyll.I have tried the CTAB,Qiagen mini kit,DNAzol and the
Dellaporta methods.I have got good yields from control tissues but not
much DNA from the ethanol soaked tissues.I would like to know if and how
DNA can be extracted from ethanol soaked plant material.




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