Extraction of RNA from heart tissue?

ELADER at AMBION.COM ELADER at AMBION.COM
Fri Jul 31 22:22:27 EST 1998


In article <6pqcb3$nnh$1 at artemis.backbone.ou.edu>,
  frank at omrf.ouhsc.edu (Bart Frank) wrote:
> Are there any precautions for extracting RNA from heart tissue that are unique
> to that tissue? I am planning on using guanidinium thiocyanate. Is RNAse a big
> problem with heart muscle as it is with pancreas, for example?

GiTC is fine. Polytron it fresh or snap freeze and grind to a powder. Don't
try to dounce it.

Eric
Ambion, Inc.

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