Plasmid Recovery From Yeast

[Frederik Boernke]

Fraser Dr N J wrote:
> 
> A colleague is having problems recovering plasmid from yeast after a
> 2-hybrid screen for transformation into E. coli. Does anyone have a
> preferred method for doing this that works routinely ?
> Thanks - Neil.

Hi!

Try the method published by Hoffmann and Winston, Gene
1987;57(2-3):267-272. This alway worked perfectly for me.
Or search the bionet archive at www.bio.net, there you'll
find loads of suggestions concerning this topic.

hope this helps
Ricky

******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiologie
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
e-mail: boernke at ipk-gatersleben.de
http://www.ipk-gatersleben.de