Plasmid Recovery From Yeast

Michael J. Prigge prigge at darkwing.uoregon.edu
Mon Jun 1 21:02:20 EST 1998


In article <357267C7.41C6 at ggr.co.uk>, Fraser Dr N J <njf16154 at ggr.co.uk> wrote:

> A colleague is having problems recovering plasmid from yeast after a
> 2-hybrid screen for transformation into E. coli. Does anyone have a
> preferred method for doing this that works routinely ?
> Thanks - Neil.

I also know of someone having the same problems. He made primers that
flank the insert and PCR amplified it. The unique positives' PCR products
were sequenced directly. Of course, it'd be nice to have the whole
plasmid, but at least he got some information in the meantime.
Mike

Michael Prigge                          Phone: (541) 346-4256
Institute of Molecular Biology            Fax: (541) 346-5891
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