Immunodetection of 40-50kDa proteins

Jose C. Juarez jcjuarez at
Mon Jun 1 14:55:05 EST 1998

I had an antigen in the same region where the IP Ab would migrate. What
helped in the end was titrating out the IP Ab so that I could get the
background signal much lower than my positive controls. I think I ended up
IPing with about 0.2 micrograms Ab per 500-1000 ug of cell lysates.

More information about the Methods mailing list