cos7 cells

karen straube-west straubek at VAX.CS.HSCSYR.EDU
Tue Jun 2 12:41:11 EST 1998


This may not be the source of your colleague's problem, but I recently
had an extended conversation with a salesperson from Krackler concerning
the Corning/Costar merger. It seems that there is no rhyme or reason to
which facilities are making which type of plates, flasks, etc. This came
up because someone in our lab ordered new 96 well plates and suddenly
had lots of dead and floating cells. It turns out that, in this case,
the new plates were supplied from Costar and she had used Corning (or
vice versa, I can't remember), even though it wasn't obvious in the
catalog. They were able to supply her with enough of the old plates so
that she could finish up her current experiments (for her thesis, no
less!). We were also informed that we have not been the only ones with
this problem. 

I have myself noticed that certain cell types are very finicky with
regard to the type of dish, flask, or even coverslip they will grow on.
Cells that grow well on Corning plates, for example, may not do well on
Costar or Falcon plates. The reasons aren't always clear, however some
manufacturing processes can leave behind residue that the cells don't
like. Your colleague may wish to check to see if they have recently
received new shipments of plasticware that could be the source of the

Mr. G. Morley wrote:
>  Hello all, a colleague of mine is having difficukty culturing some
> COS7     cells. The cells are not prolliferating well in media and I was
> wondering if anyone had any ideas or encountered the same problem.
> It is unlikely that  the foetal calf serum is  at fault as the problem has manifested
> itself quite recently and  the fcs batch has not chabged.
> I would be obligued at any ideas,
> thanks in advance.....
> gary morley

Karen Straube-West, Ph.D.
VA Medical Center
Research Service 151S
800 Irving Avenue
Syracuse, NY 13210

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