COS-7 transfection

marc.bolliger at bc.biol.ethz.ch marc.bolliger at bc.biol.ethz.ch
Tue Jun 2 10:35:34 EST 1998


I have a problem getting stable transfected COS-7 cells. Additionally to the
vector with my gene of interest, I co-transfected the cells with the selection
vector containing the neomycin resistance gene. My question is: At what
concentration do I have to use geneticin (G418 sulphate) to get stable clones?
I applied 500 microgram/ml, but no selection was observed.

Thanks in advance
Marc

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