50kb cosmids sequencing

Charlie Power genseq at tin.it
Wed Jun 3 08:32:29 EST 1998


Hi ,
does anyone knows how to sequence cosmids with 30-50 kb inserts?
I'm working on a 373ABI sequencer with Dye Terminator Chemistry, and the
reactions are made with 1500-2000 ng of DNA and 5 pm/rz of primers that
have a Tm around 60° C. PCR conditions: 94°C-10", 60°C-3', 25 cycles.
Thanks in advance

Chicco




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