Probe purification (gel shift and footprinting)
f1221 at CC.NAGASAKI-U.AC.JP
Wed Jun 3 19:11:35 EST 1998
At 3:41 PM 98.6.3, $BIr(Jic Trottier wrote:
> I'm purifying my double stranded oligoprobes from free nucleotides and
> single stranded oligo by elution after migration on a polyacrylamide gel.
> It takes a lot of time but it's clean. I need those probes for gel shift
> analysis and DNA footprinting.
> I want to know if someone use an alternative protocol, or kit, or columns
> that works faster and give a good purification. I will appreciate it
> Can I also use those alternative protocols with a probe derived from a DNA
> fragment (170 bp)?
> Thanks in advance.
you can use columns for probe purification (oligos or longer fragments).
Premade columns from 5`->3` worked well, but also you can make your
own Sephadex G50 spin columns.
Dr. T. Jakobs
University of Nagasaki
Department of Human Genetics
More information about the Methods