Probe purification (gel shift and footprinting)
erictro at CLIC.NET
Wed Jun 3 17:41:38 EST 1998
I'm purifying my double stranded oligoprobes from free nucleotides and
single stranded oligo by elution after migration on a polyacrylamide gel.
It takes a lot of time but it's clean. I need those probes for gel shift
analysis and DNA footprinting.
I want to know if someone use an alternative protocol, or kit, or columns
that works faster and give a good purification. I will appreciate it
Can I also use those alternative protocols with a probe derived from a DNA
fragment (170 bp)?
Thanks in advance.
More information about the Methods