Probe purification (gel shift and footprinting)

Éric Trottier erictro at CLIC.NET
Wed Jun 3 17:41:38 EST 1998


Hi.
I'm purifying my double stranded oligoprobes from free nucleotides and
single stranded oligo by elution after migration on a polyacrylamide gel.
It takes a lot of time but it's clean.  I need those probes for gel shift
analysis and DNA footprinting.

I want to know if someone use an alternative protocol, or kit, or columns
that works faster and give a good purification.  I will appreciate it
greatly.

Can I also use those alternative protocols with a probe derived from a DNA
fragment (170 bp)?

Thanks in advance.

Eric.





More information about the Methods mailing list