293 Transfection

delios97 at hotmail.com delios97 at hotmail.com
Wed Jun 3 17:15:44 EST 1998


I am transfectng 293 cells on poly-lysine coated plates, and am still
having cells detach.  i was woundering if anyone has any "tricks" in
preventing this from occuring.  I was considering on making a cocktail
of Fibronectin, Collagen, and Vitronectin to include in plating media.
Any suggestions would be welcomed.

Thanks




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