Using Tri Reagent to Extract Plant DNA
Mary_Wood at BAYLOR.EDU
Mary_Wood at BAYLOR.EDU
Wed Jun 3 16:36:58 EST 1998
I've been TRYING to use a product called Tri-Reagent to extract genomic DNA from
tobacco plants. Supposedly the DNA is in the interphase and phenol phase of the
mixture after removal of RNA in the aqueous phase. But I am getting very little
DNA -- about 200 ng from 100 mg of plant tissue after homogenizing in liquid
nitrogen. Has anyone used this product to extract genomic plant DNA? How did
you do it?
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