Using Tri Reagent to Extract Plant DNA

Mary_Wood at BAYLOR.EDU Mary_Wood at BAYLOR.EDU
Wed Jun 3 16:36:58 EST 1998


I've been TRYING to use a product called Tri-Reagent to extract genomic DNA from 
tobacco plants.  Supposedly the DNA is in the interphase and phenol phase of the 
mixture after removal of RNA in the aqueous phase.  But I am getting very little 
DNA -- about 200 ng from 100 mg of plant tissue after homogenizing in liquid 
nitrogen.  Has anyone used this product to extract genomic plant DNA?  How did 
you do it? 




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