Solubilizing RNA in water

Frank O. Fackelmayer fof1 at chclu.chemie.uni-konstanz.de
Thu Jun 4 01:43:31 EST 1998


Hi Mary,
Have you tried boiling the sample for 5 to 10minutes. I found that RNA
dissolves more easily in hot water than at room temp.

Hope this helps,
Frank



Mary_Wood at BAYLOR.EDU wrote:
> 
> I've been using a product called Tri-Reagent to isolate RNA from tobacco plants.
> I get large amounts of RNA but often the pellet is insoluble in water after the
> 75% ethanol wash, even when I don't dry the pellet at all.  I need to be able to
> go into an RT-PCR with my sample.  Does anyone have a solution?



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