PCR mistakes

Clemens Suter-Crazzolara, PhD un691cs at genius.embnet.dkfz-heidelberg.de
Wed Jun 3 05:46:19 EST 1998

On Wed, 03 Jun 1998 11:02:34 +0100, Richard P. Grant said:

>I found that I could get good error rates (i.e. no mistakes) with Taq by
>lowering the concentration of dNTPs, from 200 uM to 50 uM.

>Using this method, 1.9 kb frags amplified with no worries.  You may end up
>with less product, admittedly . . .

I used 50 uM as well, AND a proofreading enzyme (ULTMA), so that can't be 
the problem... Did you use simply Taq ? Could you tell some more about your conditions ?
How many cycles for instance ?

thanks clemens

More information about the Methods mailing list