Clemens Suter-Crazzolara, PhD
un691cs at genius.embnet.dkfz-heidelberg.de
Wed Jun 3 05:46:19 EST 1998
On Wed, 03 Jun 1998 11:02:34 +0100, Richard P. Grant said:
>I found that I could get good error rates (i.e. no mistakes) with Taq by
>lowering the concentration of dNTPs, from 200 uM to 50 uM.
>Using this method, 1.9 kb frags amplified with no worries. You may end up
>with less product, admittedly . . .
I used 50 uM as well, AND a proofreading enzyme (ULTMA), so that can't be
the problem... Did you use simply Taq ? Could you tell some more about your conditions ?
How many cycles for instance ?
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