Clemens Suter-Crazzolara, PhD
un691cs at genius.embnet.dkfz-heidelberg.de
Wed Jun 3 04:39:04 EST 1998
I am trying to clone a PCR product of 750bp, but it appears to
contain about 4 PCR mistakes. The polymerase I use is Ultma from
As the RNA is of pretty low abundance, I have to amplify for 32
cycles, still this wasn't much of a problem in the past.
Do you have any idea how to circumvent this high failure rate of
the PCR ? (I re-call lowering the MgCL2 concentration, currently
at 2.5 mM).
More information about the Methods