Stable Amino Acid Derivative for HPLC?
d_reeve at reeveltd.demon.co.uk
Thu Jun 4 03:51:46 EST 1998
Aren't dansyl derivatives reasonably stable? I seem to remember seeing
purified dansyl-amino acids in the Sigma catalogue.
In article <calvert-ya02408000R0306981719050001 at taco.cc.ncsu.edu>, Phil
Calvert <calvert at eos.ncsu.edu> writes
>There a number of methods available for derivatizing amino acids and
>analyzing them by reverse-phase HPLC on a C18 column. Unfortunately, many
>of these derivatives are not very stable. Obviously, they are stable
>enough for analytical purposes. But I am interested in doing preparative
>HPLC and isolating the purified amino acid derivatives; so if the
>derivatives aren't very stable, that sort of defeats the purpose.
>I would like to use a UV detector for detecting the derivatized amino
>acids. Therefore, derivatives that are UV-absorbing (including fluorescent
>derivatives) are preferred (provided that they are stable). Anyone know of
>any derivatives that fit these qualifications?
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