Solubilizing RNA in water

Frederik Boernke boernke at nospam.ipk-gatersleben.de
Thu Jun 4 12:35:12 EST 1998


Frank O. Fackelmayer wrote:
> 
> Hi Mary,
> Have you tried boiling the sample for 5 to 10minutes. I found that RNA
> dissolves more easily in hot water than at room temp.
> 
> Hope this helps,
> Frank
> 
> Mary_Wood at BAYLOR.EDU wrote:
> >
> > I've been using a product called Tri-Reagent to isolate RNA from tobacco plants.
> > I get large amounts of RNA but often the pellet is insoluble in water after the
> > 75% ethanol wash, even when I don't dry the pellet at all.  I need to be able to
> > go into an RT-PCR with my sample.  Does anyone have a solution?

Hi!

We routinely dissolve our plant RNA at 65 deg. for half an hour. 
After that centrifuge and transfer supernatant to a fresh tube. 

Ricky


******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
e-mail: boernke at ipk-gatersleben.de
http://www.ipk-gatersleben.de



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