Richard P. Grant
see_sig at cmtech.co.delete.uk
Thu Jun 4 02:15:44 EST 1998
In article <6l4q14$8gd at mserv1.dl.ac.uk>, wgschech at med.uni-tuebingen.de wrote:
: What transfection method do you use?
: I just can tell you that my 293s are sensitive, too. I'm using
: different calcium phosphate methods (BBS and HBS, with and without
: glycerol shock). Cells are cultivated on polystyrene plates without
: any previous coating.
So how are people transfecting these days? For my thesis I first tried
CaPhos, but the idea of pouring concrete over my cells did not appeal. So
I then went for a liposome-based method, which seemed far more gentle.
Richard P. Grant MA DPhil | rgrant at cmtech.co.uk
Senior R&D Scientist | work: http://www.cmtech.co.uk/
Cambridge Molecular | home: http://www.avnet.co.uk/adastra
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