293 Transfection

[Richard P. Grant]

In article <6l4q14$8gd at mserv1.dl.ac.uk>, wgschech at med.uni-tuebingen.de wrote:

: Hi. 
: 
: What transfection method do you use?
: 
: 
: I just can tell you that my 293s are sensitive, too. I'm using 
: different calcium phosphate methods (BBS and HBS, with and without 
: glycerol shock). Cells are cultivated on polystyrene plates without 
: any previous coating. 

So how are people transfecting these days?  For my thesis I first tried
CaPhos, but the idea of pouring concrete over my cells did not appeal.  So
I then went for a liposome-based method, which seemed far more gentle.

Richard

-- 
Richard P. Grant MA DPhil  | rgrant at  cmtech.co.uk
Senior R&D Scientist       | work: http://www.cmtech.co.uk/
Cambridge Molecular        | home: http://www.avnet.co.uk/adastra
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