Solubilizing RNA in water

Thibaud Le Mouël lemouel at antibes.antibes.inra.fr
Thu Jun 4 03:24:14 EST 1998


>Mary_Wood at BAYLOR.EDU wrote:
>
> I've been using a product called Tri-Reagent to isolate RNA from tobacco
>plants.
> I get large amounts of RNA but often the pellet is insoluble in water
>after the
> 75% ethanol wash, even when I don't dry the pellet at all.  I need to be
>able to
> go into an RT-PCR with my sample.  Does anyone have a solution?
>
If you want, you can try to let the RNA in water at -20°C over night, it's
better to dissolve them.

Bye
Thib


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