Solubilizing RNA in water

[Clemens Suter-Crazzolara, PhD]

On Thu, 04 Jun 1998 08:43:31 +0200, Frank O. Fackelmayer  said:

>Hi Mary,
>Have you tried boiling the sample for 5 to 10minutes. I found that RNA
>dissolves more easily in hot water than at room temp.

That may also lead to degradation, either by RNAses, or pH problems.
Check that the pH of the RNA solution is about 8. Then heat at 55C for 10 minutes.

clemens