Solubilizing RNA in water

Clemens Suter-Crazzolara, PhD un691cs at genius.embnet.dkfz-heidelberg.de
Thu Jun 4 02:31:38 EST 1998


On Thu, 04 Jun 1998 08:43:31 +0200, Frank O. Fackelmayer  said:

>Hi Mary,
>Have you tried boiling the sample for 5 to 10minutes. I found that RNA
>dissolves more easily in hot water than at room temp.

That may also lead to degradation, either by RNAses, or pH problems.
Check that the pH of the RNA solution is about 8. Then heat at 55C for 10 minutes.

clemens
 




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