cyanogen bromide cleavage
atlantis at netcom.com
Thu Jun 4 14:49:57 EST 1998
Jonathan Anderson (JAnderson at botany.uq.edu.au) wrote:
: Does anyone know if a realtively high (300mM) concentration of NaCl has
: any effect on cyanogen bromide cleavage???
I think free alkalies react with CNBr to form alkali bromide and alkali
cyanide. i.e. You often dispose of excess CNBr by mixing with dilute
NaOH. I don't know if 300mM is enough, but it probably reduces the
amount of CNBr reacting with protein so that there might not be enough
(or any) left.
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