cyanogen bromide cleavage

JJ Miranda atlantis at
Thu Jun 4 14:49:57 EST 1998

Jonathan Anderson (JAnderson at wrote:
: Does anyone know if a realtively high (300mM) concentration of NaCl has 
: any effect on cyanogen bromide cleavage???

I think free alkalies react with CNBr to form alkali bromide and alkali 
cyanide.  i.e. You often dispose of excess CNBr by mixing with dilute 
NaOH.  I don't know if 300mM is enough, but it probably reduces the 
amount of CNBr reacting with protein so that there might not be enough 
(or any) left.

Sincere regards,
JJ Miranda

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