Stable Amino Acid Derivative for HPLC?

Marco van de Weert aweert at wxs.nl
Thu Jun 4 13:22:11 EST 1998


Phil Calvert wrote:
> 
> There a number of methods available for derivatizing amino acids and
> analyzing them by reverse-phase HPLC on a C18 column.  Unfortunately, many
> of these derivatives are not very stable.  Obviously, they are stable
> enough for analytical purposes.  But I am interested in doing preparative
> HPLC and isolating the purified amino acid derivatives; so if the
> derivatives aren't very stable, that sort of defeats the purpose.
> 
> I would like to use a UV detector for detecting the derivatized amino
> acids.  Therefore, derivatives that are UV-absorbing (including fluorescent
> derivatives) are preferred (provided that they are stable).  Anyone know of
> any derivatives that fit these qualifications?
> 
> Phil Calvert

AFAI remember, derivatizing them with ortho-phtalaldehyde (the OPA
reagent, or fluoraldehyde) is reasonably stable

Marco





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