competent cells again!!

gwallmanning at gwallmanning at
Thu Jun 4 16:24:15 EST 1998

In article <6l4qb0$8rj at>,
  wgschech at wrote:
> Do you have to
> dilute it before or does 100% glycerol go through the membranes?
I normally mix up a solution of 100 mM CaCl2 with 15% glycerol and filter
sterilize that.  I have never tried to filter 100% glycerol.  The thought of
trying to force THAT stuff through a membrane makes me go all weak! :-)

If you try it, let me know how it goes!



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