competent cells again!!

[gwallmanning at wnmeds.ac.nz]

In article <6l4qb0$8rj at mserv1.dl.ac.uk>,
  wgschech at med.uni-tuebingen.de wrote:
>
> Do you have to
> dilute it before or does 100% glycerol go through the membranes?
>
I normally mix up a solution of 100 mM CaCl2 with 15% glycerol and filter
sterilize that.  I have never tried to filter 100% glycerol.  The thought of
trying to force THAT stuff through a membrane makes me go all weak! :-)

If you try it, let me know how it goes!

Cheers,

Glenn

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