Deionized Formamide - A Mystery?

theodorn at medlib.georgetown.edu theodorn at medlib.georgetown.edu
Fri Jun 5 17:44:44 EST 1998


In article <6l9asc$5g3$2 at supernews.com>,
  meyerdj at phibred.com (Dr. David J. Meyer) wrote:
>
> What happens when formamide is deamidated? Answer: you get formic acid
> and ammonia!
>
>

And those of us old enough to remember Maxim-Gilbert sequencing may recall
that formic acid was used for one of the base-modification reactions (was it
G? or G+ A? - gee, it's been so long)

Nick Theodorakis




 JKGH at LELA.COM (GH) wrote:
>
> >Hi, I'm not sure exactly why it is important. It is probably to remove
> >other ions. The RNA loading buffer is at least 50% formamide so it is the
> >> >In article <6khams$r1s at mserv1.dl.ac.uk>, wgschech at med.uni-tuebingen.de wrote:
<snip>
> >> Hi all,
> >>
> >> Why is it so important for formamide to be deionized? Especially when
<snip>

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