Solubilizing RNA in water
Clemens Suter-Crazzolara, PhD
un691cs at genius.embnet.dkfz-heidelberg.de
Fri Jun 5 02:52:09 EST 1998
On 4 Jun 1998 06:21:55 -0700, Michael Allen said:
>Recently a colleague who has moved on e-mailed me to say that in her new
>lab they routinely freeze their homogenized tissue in the trireagent
>overnight at -80. Proceeding with the protocol after this freezing step
>drastically reduces polysaccharide precipitation, at least in their hands.
It also reduces the amount of RNA recovered !
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