Solubilizing RNA in water

[Clemens Suter-Crazzolara, PhD]

On 4 Jun 1998 06:21:55 -0700, Michael Allen said:

>Hi Mary
>Recently a colleague who has moved on e-mailed me to say that in her new 
>lab they routinely freeze their homogenized tissue in the trireagent
>overnight at -80. Proceeding with the protocol after this freezing step
>drastically reduces polysaccharide precipitation, at least in their hands.

It also reduces the amount of RNA recovered !

clemens