RIPPA kit for RPA

Gail Otulakowski gotulak at sickkids.on.ca
Fri Jun 5 13:58:01 EST 1998


Has anyone tried the new RNAse protection proximity assay (RiPPA) kit
from Amersham yet, or a similar homemade method?  Opinions?  The speed,
and use of biotin and 33P instead of 32P are tempting.  However, I've
had considerable trouble in the past getting a specific protected band
from RPA (Maybe because my probe, to transcription start region, is
GC-rich).  Without a gel step, in RiPPA, how would you know that your
result is specific??

Gail Otulakowski



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